偏最小二乘-分光光度法同时测定芦丁与槲皮素的方法研究

芦丁和槲皮素在紫外区的最大吸收波长接近,吸收光谱严重重叠,普通光度法分析难以实现两者的直接测定.该文通过测定以1∶4甲醇-水为溶剂的芦丁、槲皮素标准混合溶液在200～500 nm波长范围的吸收光谱,采用偏最小二乘法(PLS法)建立校正模型,对样品中的芦丁和槲皮素的含量进行预测,建立了偏最小二乘-分光光度法同时测定芦丁和...

Simultaneous Determination of Rutin and Quercetin by Partial Least Squares-Spectrophotometry

The maximum absorption wavelengths of rutin and quercetin are very close and their absorption spectra are seriously overlapping,so it’s very difficult to determine individually rutin or quercetin by spectrophotometry without prior separation.In this paper,a partial least squares-spectrophotometry was developed for the simultaneous determination of rutin and quercetin.The absorption spectra of rutin and quercetin mixed standard were studied in the range of 200-500 nm and the absorbance values were recorded every 2 nm.A partial least-squares(PLS) model was built for the simultaneous determination of the contents of rutin and quercetin in the flos sophorae and synthetic samples.The results indicated that the recoveries of rutin and quercetin in the flos sophorae were in the range of 95.6%-99.8% and 99.8%-99.9%,respectively,and the recoveries of rutin and quercetin in the synthetic samples were in the range of 97.6%-102.1% and 100.2%-102.2%,respectively.The method showed high accuracy and satisfied precision,and could reduce the effect of noise effectively.