| 胶内差异双向电泳-电喷雾串联质谱筛选UVB损伤皮肤细胞潜在的蛋白质靶标 |
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| 引用本文： | 孙明忠,郑体花,徐康,张春艳,杨帆,田余祥,刘淑清.胶内差异双向电泳-电喷雾串联质谱筛选UVB损伤皮肤细胞潜在的蛋白质靶标[J].分析测试学报,2011,30(4):356-361. |
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| 作者姓名： | 孙明忠 郑体花 徐康 张春艳 杨帆 田余祥 刘淑清 |
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| 作者单位： | 1.大连医科大学生物技术系；2.大连医科大学生物化学教研室 |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目,国家自然科学基金资助项目（81050010），辽宁教育厅重点实验室资助项目（LS2010050） |
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| 摘 要： | 联用胶内差异双向电泳(2D-DIGE)和高效液相色谱-电喷雾串联质谱(HPLC-nESI MS/MS)鉴定人角质形成细胞HaCaT应答中波紫外线(UVB)损伤的差异表达蛋白,筛选UVB影响皮肤细胞正常生理功能潜在的靶标蛋白.结果表明:UVB辐射明显影响HaCaT细胞的蛋白质表达谱,DeCyder软件在每块DIGE凝胶上...
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| 关 键 词： | UVB HaCaT细胞 胶内差异双向凝胶电泳 高效液相色谱-电喷雾质谱联用 |
Identification of Potential Protein Biomarkers for Skin Cell Damaged by UVB Irradiation Using 2D DIGE Coupled to HPLC-nESI MS/MS |
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| SUN Ming-zhong,ZHENG Ti-hua,XU Kang,ZHANG Chun-yan,YANG Fan,TIAN Yu-xiang,LIU Shu-qing.Identification of Potential Protein Biomarkers for Skin Cell Damaged by UVB Irradiation Using 2D DIGE Coupled to HPLC-nESI MS/MS[J].Journal of Instrumental Analysis,2011,30(4):356-361. |
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| Authors: | SUN Ming-zhong ZHENG Ti-hua XU Kang ZHANG Chun-yan YANG Fan TIAN Yu-xiang LIU Shu-qing |
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| Institution: | SUN Ming-zhong1,ZHENG Ti-hua2,XU Kang2,ZHANG Chun-yan1,YANG Fan2,TIAN Yu-xiang2,LIU Shu-qing2(1.Department of Biotechnology,Dalian Medical University,Dalian 116044,China,2.Department of Biochemistry,China) |
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| Abstract: | Two-dimensional difference in gel electrophoresis(2D-DIGE) combined with high performance liquid chromatography-nano-electron spray ionization tandem mass spectrometric(HPLC-nESI MS/MS) method was developed for the identification of the differentially expressed potential protein markers of human keratinocytes cell HaCaT in response to UVB irradiation,which aims to obtain the target proteins affecting the normal physiological function of skin cells induced by UVB irradiation.The results indicated that UVB ir... |
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| Keywords: | UVB HaCaT two-dimensional difference in gel electrophoresis HPLC-nESI MS/MS |
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