有机磷药物在水产品中的存在状态研究及其分析应用

用分子荧光法、气相色谱法探讨了有机磷药物在以蛋白质为主要基质的水产品中的存在状态, 并研究了蛋白质对有机磷药物提取的影响. 结果表明, 有机磷药物与蛋白质有较强结合作用. 体积分数为70%～90%乙腈水溶液可引起蛋白质变性伸展, 利于释放结合的药物, 从而提高蛋白质基质中的有机磷提取回收率. 以体积比乙腈∶乙酸∶水＝90∶1∶9的溶液提取鱼肉样品中的有机磷药物, 以PSA, GDX-103为混合颗粒吸附剂, 以无水硫酸镁、无水醋酸钠为混合无机盐除水剂, 经离心分离去除基质后萃取液直接进样测定了鱼肉中5种有机磷药物(敌敌畏、甲胺磷、乐果、甲基对硫磷和乙基对硫磷), 测定回收率为72.97%～107.21%, 检出限为0.002～0.045 mg•kg^－1. 方法操作步骤简单、快速、高效.

Study on the Form of Organic Phosphorus in Aquatic Products and Its Analytical Application

Molecular fluorescence spectrometry and gas chromatography were applied to study the form of organic phosphorus in protein matrix of aquatic products.The influence of proteins on extraction of organic phosphorus from aquatic products samples was also discussed.The results showed that there was a strong binding effect between organic phosphorus and proteins.In the volume fraction of acetonitrile(ACN) be-tween 70% and 90% aqueous solution protein denaturation could occur and the organic phosphorus binding wit...