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| PVP两亲性嵌段共聚物对超螺旋DNA的裂解活性 | |
| 引用本文： | 张立芬,梁勇,孟令芝. PVP两亲性嵌段共聚物对超螺旋DNA的裂解活性[J]. 化学学报, 2011, 69(16): 1941-1945 |
| 作者姓名： | 张立芬 梁勇 孟令芝 |
| 作者单位： | 1. 兰州大学化学化工学院,兰州730000／武汉大学化学与分子科学学院,武汉430072 2. 江汉大学医学与生命科学学院,武汉,430056 3. 武汉大学化学与分子科学学院,武汉,430072 |
| 基金项目： | 国家自然科学基金(No.20644002); 教育部博士点基金(No.20060486027); 兰州大学中央高校基本科研业务费专项(No.lzujbky-2009-27)和兰州大学交叉学科青年创新研究基金(No.LZUJC200911)资助项目 |
| 摘 要： | 以PVP-Cl为大分子引发剂,经ATRP聚合制备得到窄分子量分布、结构可控的含不同亲水/疏水比例的两亲性聚N-乙烯基吡咯烷酮-b-聚N-甲基丙烯酰基-N＇-Boc-色氨酸酰胺基硫脲嵌段共聚物（PVP-b-PTrpAMT-I,II,III）.1H NMR核磁共振、GPC-MALLS以及凝胶电泳对嵌段共聚物的结构进行了表征... |
| 关 键 词： | 聚(N-乙烯基吡咯烷酮) 嵌段共聚物 DNA 裂解活性 |
Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA |
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| Zhang,Lifen,a,b Liang,Yongc Meng,Lingzhib. Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA[J]. Acta Chimica Sinica, 2011, 69(16): 1941-1945 | |
| Authors: | Zhang Lifen a b Liang Yongc Meng Lingzhib |
| Affiliation: | Zhang,Lifen,a,b Liang,Yongc Meng,Lingzhib(aCollege of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000)(bCollege of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072) (c School of Medicine and Life Science,Jianghan University,Wuhan 430056) |
| Abstract: | Amphiphilic block copolymers poly(N-vinylpyrrolidone)-block-poly(N-t-Boc-tryptophanamido-N'-methacryl thiourea)(PVP-b-PTrpAMT-I,II,III) were designed and synthesized with well defined structures by atom transfer radical polymerization(ATRP) using PVP-Cl as macroinitiator.The chemical structures of the block copolymers were characterized by 1H NMR and GPC-MALLS.Agarose gel electrophoresis study demonstrated that PVP-b-PTrpAMT can induce and cleavage supercoiled DNA into nicked DNA.The nicked DNA can avoid th... |
| Keywords: | poly(N-vinylpyrrolidone) block copolymer DNA cleavage activity |
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