Effects of hemicellulose and lignin on enzymatic hydrolysis of cellulose from dairy manure |
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Authors: | Wei Liao Zhiyou Wen Sharon Hurley Yan Liu Chuanbin Liu Shulin Chen |
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Institution: | (1) Department of Biological Systems Engineering and Center for Multiphase Environmental Research, Washington State University, 99163 Pullman, WA;(2) Department of Chemistry, Jackson State University, 39217 Jackson, MS |
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Abstract: | This study focused on the effect of hemicellulose and lignin on enzymatic hydrolysis of dairy manure and hydrolysis process
optimization to improve sugar yield. It was found that hemicellulose and lignin in dairy manure, similar to their role in
other lignocellulosic material, were major resistive factors to enzymatic hydrolysis and that the removal of either of them,
or for best performance, both of them, improved the enzymatic hydrolysis of manure cellulose. This result combined with scanning
electron microscope (SEM) pictures further proved that the accessibility of cellulose to cellulase was the most important
feature to the hydrolysis. Quantitatively, fed-batch enzymatic hydrolysis of fiber without lignin and hemicellulose had a
high glucose yield of 52% with respect to the glucose concentration of 17 g/L at a total enzyme loading of 1300 FPU/L and
reaction time of 160 h, which was better than corresponding batch enzymatic hydrolysis. |
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Keywords: | Cellulose fed-batch enzymatic hydrolysis glucose hemicellulose yield |
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