Capture of cationic ligands bound diffusely to base pairs during DNA refolding |
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| Authors: | Nakano Shu-ichi Kirihata Toshimasa Sugimoto Naoki |
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| Affiliation: | Frontier Institute for Biomolecular Engineering Research, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan. |
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| Abstract: | We obtained the intrinsic binding affinity for metal ions, polyamines, and oligolysine peptides diffusely bound to base-paired sites in DNA by monitoring the shift of the hairpin-duplex equilibrium of the self-complementary DNA sequences, which can be widely used for capturing cationic ligands bound diffusely to nucleotide base pairs. |
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