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Quantitative determination of oxidized carbon nanotube probes in yeast by capillary electrophoresis with laser-induced fluorescence detection |
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| Authors: | Xiao Hua Zou Hanfa Pan Chensong Jiang Xiaogang Le X Chris Yang Ling |
| Affiliation: | aNational Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China bDepartment of Public Health Sciences, Faculty of Medicine, University of Alberta, 10-102 CSB, Edmonton, Alberta T6G 2G3, Canada cLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
| Abstract: | Short oxidized multi-walled carbon nanotubes were functionalized with fluorescein isothiocyanate to form carbon nanotube probes (CNTP). The distribution of CNTP in yeast was quantitatively determined by capillary electrophoresis coupled with laser-induced fluorescence detection. The detection sensitivity for CNTP was greatly improved comparing with UV absorbance and Raman detection. The time- and temperature-dependent influx patterns of CNTP into yeast were obtained. The apparent permeability coefficient for influx of CNTP into yeast was calculated, which suggested that CNTP might permeate into yeast through endocytosis. This study implies that CNTP could be a fine drug transporter and might be wildly used in multidrug resistance research and microorganism detection. |
| Keywords: | Capillary electrophoresis Laser-induced fluorescence detection Carbon nanotube probes Yeast Apparent permeability coefficient |
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