Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection |
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Authors: | Neil L. Kelleher Michael W. Senko Daniel P. Little Peter B. O’Connor Fred W. McLafferty |
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Affiliation: | 1. Department of Chemistry, Cornell University, Ithaca, New York, USA
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Abstract: | For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5×10?14 mol (0.5 µL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 µs from a 0.15-µL/min injection of 20-µM ubiquitin consumes 5×10?18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and τ;3×105 resolving power. |
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