Separation of natural product using columns packed with Fused‐Core particles |
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Authors: | Peilin Yang George R Litwinski Matthias Pursch Terry McCabe Krishna Kuppannan |
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Institution: | 1. The Dow Chemical Company, Analytical Sciences, Midland, MI, USA. Fax: +1‐989‐636‐8450;2. Dow Deutschland GmbH, Analytical Sciences, Rheinmuenster, Germany |
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Abstract: | Three HPLC columns packed with 3 μm, sub‐2 μm, and 2.7 μm Fused‐Core (superficially porous) particles were compared in separation performance using two natural product mixtures containing 15 structurally related components. The Ascentis ExpressTM C18 column packed with Fused‐Core particles showed an 18% increase in column efficiency (theoretical plates), a 76% increase in plate number per meter, a 65% enhancement in separation speed and a 19% increase in back pressure compared to the Atlantis T3TM C18 column packed with 3 μm particles. Column lot‐to‐lot variability for critical pairs in the natural product mixture was observed with both columns, with the Atlantis T3 column exhibiting a higher degree of variability. The Ascentis Express column was also compared with the AcquityTM BEH column packed with sub‐2 μm particles. Although the peak efficiencies obtained by the Ascentis Express column were only about 74% of those obtained by the Acquity BEH column, the 50% lower back pressure and comparable separation speed allowed high‐efficiency and high‐speed separation to be performed using conventional HPLC instrumentation. |
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Keywords: | Ascentis Express C18 Fused‐Core column technology Natural product Sub‐2 μ m particles |
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