Zwitterionic molecularly imprinted polymer‐based solid‐phase micro‐extraction coupled with molecularly imprinted polymer sensor for ultra‐trace sensing of L‐histidine |
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Authors: | Bhim Bali Prasad Khushaboo Tiwari Meenakshi Singh Piyush S Sharma Amit K Patel Shrinkhala Srivastava |
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Institution: | Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India. Fax: +91 5422 368174 |
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Abstract: | The proposed L ‐histidine sensing system composed of a molecularly imprinted solid‐phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non‐specific sorption and false‐positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid‐phase microextraction material. The hyphenated approach was able to detect L ‐histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3). |
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Keywords: | L‐Histidine Human blood serum Molecularly imprinted polymer brush Molecularly imprinted polymer‐sensor Solid‐phase microextraction |
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