Stabilizer‐Free Poly(lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots as a Potential Carrier Applied in Human Mesenchymal Stem Cells |
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| Authors: | Wen‐Shuo Kuo Shiaw‐Min Hwang Hei‐Tin Sei Yu‐Cian Ku Lee‐Feng Hsu Fong‐Yu Cheng Patrick Ching‐Ho Hsieh Chen‐Sheng Yeh |
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| Institution: | 1. Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan, R.O.C.;2. Food Industry Research and Development Institute, Hsinchu 300, Taiwan, R.O.C.;3. Institute of Clinical Medicine & Research Center of Clinical Medicine, National Cheng Kung University & Hospital, Tainan 701, Taiwan, R.O.C. |
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| Abstract: | We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactide‐co‐glycolide) nanoparticles (PLGA NPs) surface‐conjugated quantum dots (QDs) (PLGA‐QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA‐QD NPs at high concentrations for at least four weeks. Besides, PLGA‐QD NPs‐labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC‐loaded PLGA‐QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA‐QD NPs. In addition to PLGA‐QD NPs, QDs alone were used to serve as a control set for comparison. |
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| Keywords: | Poly(lactide‐co‐glycolide) nanoparticles Human mesenchymal stem cells Quantum dots |
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