Visual DNA as a diagnostic tool |
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Authors: | Erik Pettersson Patrik L Ståhl Hovsep Mahdessian Max Käller Joakim Lundeberg Afshin Ahmadian |
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Institution: | Division of Gene Technology, AlbaNova University Center, Stockholm, Sweden |
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Abstract: | We report on the incorporation of the Visual DNA concept in a genotyping assay as a simple and straightforward detection tool. The principle of trapping streptavidin‐coated superparamagnetic beads of micrometer size for visualization of genetic variances is used for PrASE‐based detection of a panel of mutations in the severe and common genetic disorder of cystic fibrosis. The method allows a final investigation of genotypes by the naked eye and the output is easily documented using a regular hand‐held device with an integrated digital camera. A number of samples were run through the assay, showing rapid and accurate detection using superparamagnetic beads and an off‐the‐shelf neodymium magnet. The assay emphasizes the power of Visual DNA and demonstrates the potential value of the method in future point‐of‐care tests. |
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Keywords: | Cystic fibrosis Diagnostics Mutation PrAse Visual DNA |
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