Quantification of D‐Asp and D‐Glu in rat brain and human cerebrospinal fluid by microchip electrophoresis |
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Authors: | Yong Huang Ming Shi Shulin Zhao |
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Institution: | College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China. Fax: +86‐773‐5832294 |
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Abstract: | A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D ‐aspartic acid (D ‐Asp) and D ‐glutamate (D ‐Glu) in biological samples. D ‐Asp and D ‐Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using γ‐CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D ‐Asp and D ‐Glu were 6.0×10–8 and 4.0×10–8 M, respectively. Using this method, the levels of D ‐Asp and D ‐Glu in rat brain and human cerebrospinal fluid (CSF) were determined. |
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Keywords: | D‐Aspartic acid D‐Glutamate Human cerebrospinal fluid Laser induced fluorescence Microchip electrophoresis Rat brain |
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