| 溶解-沉淀可逆的酶载体的合成及其应用 |
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| 引用本文： | 柏正武,卓仁禧.溶解-沉淀可逆的酶载体的合成及其应用[J].武汉大学学报(理学版),1998(4). |
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| 作者姓名： | 柏正武 卓仁禧 |
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| 作者单位： | 武汉大学化学学院教育部生物医用高分子材料开放实验室 |
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| 摘 要： | 由丙烯酸、甲基丙烯酸甲酯和顺丁烯二酸酐共聚，合成了ｐＨ敏感的溶解－沉淀可逆的共聚物，并测试了共聚物溶解－沉淀相分离的临界ｐＨ值．以这种共聚物为载体，进行了木瓜蛋白酶的固定化．
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| 关 键 词： | pH敏感高分子，可逆溶解－沉淀，固定化酶 |
SYNTHESIS AND APPLICATION OF pH SENSITIVE IMMOBILIZED ENZYME CARRIERS WITH REVERSIBLE SOLUBILITY PRECIPITATION PROPERTY |
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| Bai Zhengwu,Zhuo Renxi.SYNTHESIS AND APPLICATION OF pH SENSITIVE IMMOBILIZED ENZYME CARRIERS WITH REVERSIBLE SOLUBILITY PRECIPITATION PROPERTY[J].JOurnal of Wuhan University:Natural Science Edition,1998(4). |
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| Authors: | Bai Zhengwu Zhuo Renxi |
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| Abstract: | pH sensitive terpolymers with reversible solubility precipitation property when the pH values of the solution were changed were synthesized by terpolymerization of acrylic acid, methyl methacrylate and maleic anhydride. The chemical structure of these terpolymers obtained was characterized by IR spectrum. The critical pH values of solubility precipitation of these terpolymers were investigated. Immobilization of papain on these polymers were also carried out. |
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| Keywords: | pH sensitive polymer reversible solubility precipitation immobilized enzyme |
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