Solvent optimization in normal-phase liquid chromatography of selected steroids |
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| Authors: | P. E. Antle |
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| Affiliation: | (1) Analytical Instruments Division, E. I. du Pont de Nemours & Co. Inc., Concord Plaza/Quillen Building, 19898 Wilmington, DE, USA |
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| Abstract: | Summary Resolution (Rs) is a function of three factors: efficiency, retentivity and selectivity. A procedure for optimization of mobile phase selectivity was demonstrated using a reversed-phase (RP) separation of compounds with various functionalities. In this paper, the same procedure is used with normal bonded-phase (NP) chromatography to develop a separation of structurally similar steroids. The optimization of mobile-phase selectivity provided increased problem-solving capability and decreased the analysis time.A brief summary of this work has been published in September 1981, in the form of a du Pont Application Study. |
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| Keywords: | Liquid chromatography Solvent selectivity Steroids Amine bonded phase Overlapping resolution mapping |
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