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Speciation of iron in breast milk and infant formulas whey by size exclusion chromatography-high performance liquid chromatography and electrothermal atomic absorption spectrometry |
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| Authors: | Bermejo P Peña E Domínguez R Bermejo A Fraga J M Cocho J A |
| Institution: | a Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avda de las Ciencias, 15705 Galeras SN, Spain b Department of Pediatrics, Faculty of Medicine, University of Santiago de Compostela, Galeras SN, 15705 Santiago de Compostella, Spain c Laboratory of Metabolic Disorders, Complejo Universitario, University of Santiago de Compostela, Galeras SN, 15705 Santiago de Compostela, Spain |
| Abstract: | Speciation of iron in milk was carried out by high performance liquid chromatography (HPLC) and electrothermal atomic absorption spectrometry (ETAAS). Milk whey was obtained and low molecular weight protein separation was performed by size exclusion chromatography (SEC) with a TSK Gel SW glass guard (Waters) pre-column and a TSK-Gel G2000 glass (Toso Haas) column. After studying water as a possible mobile phase, this mobile phase was carefully selected in order to avoid alterations of the sample and to make subsequent iron determination in the protein fractions easier by ETAAS. The proposed method is sensitive (limit of detection LOD] and LOQ 1.4 and 4.7 μg l−1, respectively) and precise (relative standard deviation RSD]<10%). Iron is principally found in the proteins of 3 and 76 kDa in breast milk, and it is irregularly distributed in infant formulas. |
| Keywords: | Iron speciation Milk SEC-HPLC ETAAS |
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