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Determination of Spectinomycin in Human Urine Using CE Coupled with Electrogenerated Chemiluminescence |
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| Authors: | Wei Wei Wei Min Cai Zeyu Liu Songqin |
| Institution: | 1.College of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210009, People’s Republic of China ;2.College of Food Science and Technology, Henan University of Technology, Zhengzhou, 450052, People’s Republic of China ;3.Gulou Center for Disease Control and Prevention, Nanjing, 210009, People’s Republic of China ; |
| Abstract: | A method of capillary electrophoresis with electrogenerated chemiluminescence was developed for the detection of spectinomycin. The linear ranges were from 0.01 to 1.0 mg mL−1 for spectinomycin. The limit of detection for spectinomycin with a signal-to-noise ratio of 3:1 was found to be 4.0 µg mL−1. For practical application perchloric acid was used to eliminate the protein contained in human urine, which is very harmful to electrophoresis separation. The recoveries of spectinomycin at different levels in human urine were between 91.1 and 106.5%. The RSD was between 2.6 and 4.8% (n = 5–6). It was valuable in clinical and biochemical laboratories for monitoring the drug for various purposes. |
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