Turn‐On Ratiometric Fluorescent Probe for Selective Discrimination of Cr3+ from Fe3+ in Aqueous Media for Living Cell Imaging |
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| Authors: | Il Seung Youn Taeseung Yoon Dr. Kwang‐Youn Kim Dr. Young‐Kyo Seo Genggongwo Shi Muhammad Saleh Jin‐Hoe Hur Prof. Dr. Kwang S. Kim |
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| Affiliation: | 1. Center for Superfunctional Materials, Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689‐798 (Republic of Korea);2. Department of Chemistry, Pohang University of Science and Technology, Pohang, 790‐784 (Republic of Korea);3. School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689‐798 (Republic of Korea);4. UNIST Central Research Facilities (UCRF), Ulsan National Institute of Science and Technology, Ulsan 689‐798 (Republic of Korea) |
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| Abstract: | Pyrene‐based turn‐on ratiometric fluorescent probe 1 demonstrates high sensitivity and exceptional selectivity toward Cr3+ in the presence of other metals, including Fe3+ in aqueous media. Interaction of Cr3+ with probe 1 brings pyrene moieties close enough to have better aligned π–π stacking, thus enhancing the excimer peak many fold. On the other hand, the interaction of Fe3+ with probe 1 brings forth a negligible difference in stacking, resulting in an insignificant change in fluorescence intensity. Exceptional selectivity of probe 1 with Cr3+ over Fe3+ and other metals has been confirmed by theoretical studies in addition to experimental results. Imaging of HeLa cells observed by confocal fluorescence microscopy reveals that probe 1 can be used to monitor Cr3+ in live cells to map its subcellular distribution. |
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| Keywords: | chromium density functional theory fluorescence pyrene ratiometric |
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