Sensing Protein Surfaces with Targeted Fluorescent Receptors |
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Authors: | Yael Nissinkorn Naama Lahav‐Mankovski Dr Aharon Rabinkov Dr Shira Albeck Dr Leila Motiei Dr David Margulies |
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Institution: | 1. Department of Organic Chemistry, Weizmann Institute of Science, 76100, Rehovot (Israel);2. Department of Biological Services, Weizmann Institute of Science, 76100, Rehovot (Israel);3. Israel Structural Proteomics Center, Weizmann Institute of Science, 76100, Rehovot (Israel) |
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Abstract: | A methodology for creating fluorescent molecular sensors that respond to changes that occur on the surfaces of specific proteins is presented. This approach, which relies on binding cooperatively between a specific His‐tag binder and a nonspecific protein‐surface receptor, enabled the development of a sensor that can track changes on the surface of a His‐tag‐labeled calmodulin (His‐CaM) upon interacting with metal ions, small molecules, and protein binding partners. The way this approach was used to detect dephosphorylation of an unlabeled calmodulin‐dependent protein kinase II (CaMKII), and the binding of Bax BH3 to His‐tagged B‐cell lymphoma 2 (Bcl‐2) protein is also presented. |
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Keywords: | biomolecular interactions fluorescent sensors multivalency protein‐surface recognition synthetic receptors |
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