NMR Signal Enhancement by Effective SABRE Labeling of Oligopeptides |
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| Authors: | Dr Tomasz Ratajczyk Dr Torsten Gutmann Dr Piotr Bernatowicz Prof Gerd Buntkowsky Dr Jaroslaw Frydel Bartlomiej Fedorczyk |
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| Institution: | 1. Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01‐224 Warsaw (Poland);2. Technische Universit?t Darmstadt, Eduard Zintl Institut für Anorganische und Physikalische Chemie, Alarich‐Weiss‐Str. 8, 64287 Darmstadt (Germany);3. Venitur Sp. z o.o., Wawozowa 34 B, 31‐752 Krakow (Poland);4. University of Warsaw, Faculty of Chemistry, Pasteura 1, 02‐093 Warsaw (Poland) |
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| Abstract: | Signal amplification by reversible exchange (SABRE) can enhance nuclear magnetic resonance signals by several orders of magnitude. However, until now this was limited to a small number of model target molecules. Here, a new convenient method for SABRE activation applicable to a variety of synthetic model oligopeptides is demonstrated. For the first time, a highly SABRE‐active pyridine‐based biocompatible molecular framework is incorporated into synthetic oligopeptides. The SABRE activity is preserved, demonstrating the importance of such earmarking. Finally, a crucial exchange process responsible for SABRE activity is identified and discussed. |
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| Keywords: | hyperpolarization NMR spectroscopy peptides signal amplification by reversible exchange signal enhancement |
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