Bioorthogonal SERS Nanoprobes for Mulitplex Spectroscopic Detection,Tumor Cell Targeting,and Tissue Imaging |
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Authors: | Junzhou Wu Duanwei Liang Qingqing Jin Dr. Jie Liu Prof. Dr. Meiling Zheng Prof. Dr. Xuanming Duan Prof. Dr. Xinjing Tang |
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Affiliation: | 1. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, No. 38, Xueyuan Rd. Beijing 100191 (P.R. China);2. Technical Institute of Physics and Chemistry the Chinese Academy of Sciences |
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Abstract: | A surface‐enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman‐silent region of biological samples. AS1411 aptamer was also co‐conjugated with AuNF through a self‐assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small‐molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman‐silent region. These Raman nanoprobes co‐conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging. |
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Keywords: | aptamers AS1411 bioorthogonality cell targeting nanoprobes Raman spectroscopy |
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