(S)-(+)-1-Methyl-2-(6,7,-dimethoxy-2,3-naphthalimido)ethyl trifluoromethanesulfonate as a fluorescence chiral derivatizing reagent for carboxylic acid enantiomers in high-performance liquid chromatography |
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Authors: | Yuta Yasaka Yoshiharu Ono Minoru Tanaka |
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Affiliation: | Research Center for Environmental Preservation, Osaka University, Yamada-oka, Suita, Osaka 565, Japan |
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Abstract: | A new triflate-type fluorescence chiral derivatizing reagent, (S)-(+)-1-methyl-2-(6,7-dimethoxy-2,3-naphthalimido)ethyl trifluoromethanesulfonate, [S-(+)-MDNE-OTf], has been developed for the determination of the enantiomers of carboxylic acids. By introducing the two methoxy groups on the naphthalimido ring moiety, the red shift in the fluorescence spectrum and a high resolution in reversed-mode separation of the diastereomers of chiral carboxylic acids have been achieved. The detection limits (S/N=3) with ultraviolet and fluorescence detection are 8 fmol (λmax=283 nm) and 4 fmol (λex=283 nm, λem=467 nm), respectively. |
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Keywords: | Carboxylic acids |
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