Production of Bacillus sphaericus entomopathogenic biomass using brewery residues |
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Institution: | (1) Escola de Química, Centro de Tecnologia, Bloco E, Universidade Federal do Rio de Janeiro, Ilha do Fund?o, 21949-900 Rio de Janeiro, RJ, Brazil |
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Abstract: | The use of brewery residues—yeast and trub—has been evaluated aiming to minimize the costs of the industrial production of
Bacillus sphaericus-based bioinsecticide. Both brewery residues promoted growth and sporulation of the three B. sphaericus strains that were isolated from Brazilian soils (S1, S2, and S20). However, distinct growth and sporulation behaviors were
observed in relation to the different nutritional conditions and strain used. The maximum sporulation percentage was obtained
through the cultivation of S20 strain in brewery residual yeast. In general, the entomopathogenic biomasses produced showed
good results for toxicity to Culex larvae. The minimum values of larvae population (LC50) were observed for the S20 strain grown on yeast brewery residue-containing
media. After fermentation, a considerable decrease in the organic material of alternative media was verified, although the
residual values were still higher than that considered appropriate for effluent discharge. |
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Keywords: | Bacillus sphaericus bioinsecticide production industrial residues entomopathogenic biomass spore/crystal toxins |
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