Isolation and Identification of a Newly Isolated <Emphasis Type="Italic">Alternaria</Emphasis> sp. ND-16 and Characterization of Xylanase |
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| Authors: | Yin Li Zhiqiang Liu Fengjie Cui Lifeng Ping Chongyan Qiu Geng Li Lijiao Yan |
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| Institution: | (1) Department of Plant Science, North Dakota State University, Fargo, ND 58105, USA;(2) Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China;(3) NSF I/UCRC for Biocatalysis and Bioprocessing of Macromolecules, Polytechnic Institute of New York University, Six Metro Tech Center, Brooklyn, New York, 11201, USA;(4) School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 2120013, People’s Republic of China;(5) Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China;(6) School of Biotechnology, Chongqing Institute of Technology, Chongqing, 400050, People’s Republic of China;(7) College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China |
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| Abstract: | Alternaria sp. ND-16, a bacterium isolated from soil sample, was identified as a strain of Alternaria mali based on the morphology and comparison of internal transcribed spacer rDNA gene sequence studies. Furthermore, it is demonstrated that this strain has xylanase activity, and the activity can be optimized
under suitable growing conditions where wheat bran and urea are the primary sources of carbon and nitrogen. Partially purified
xylanase from Alternaria sp. ND-16 is shown to have an optimal pH of 6.0 and optimal temperature of 50 °C, making this enzyme potentially suitable
for industrial applications. It is also demonstrated that Na+ and Mn2+ show strong inhibition of the xylanase while K+, Li+, Fe2+, Cu2+, and Zn2+ have no significant effect on the activity. |
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| Keywords: | Alternaria sp ND-16 Isolation Identification Production Xylanase |
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