Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution. |
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| Authors: | F Yang T Zhang G Tian H Cao Q Liu Y Ito |
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| Affiliation: | Beijing Institute of New Technology Application, China. |
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| Abstract: | Analytical and preparative high-speed counter-current chromatography was successfully used for the isolation and purification of hydroxyanthraquinones from Rheum officinale Baill (Dahuang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion and aloe-emodin were isolated each at over 98% purity. |
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