Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases |
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| Authors: | Ito Ryousuke Mori Kouya Hashimoto Ippei Nakano Chiaki Sato Tsutomu Hoshino Tsutomu |
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| Institution: | Department of Applied Biological Chemistry, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, Nishi-ku, Niigata, Japan. |
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| Abstract: | The gene products of AK121211, AK066327, and AK070534 from Oryza sativa encode cycloartenol, parkeol, and achilleol B synthases, respectively. Parkeol synthase is a unique enzyme that affords parkeol as a single product. Achilleol B synthase is the third seco-type triterpene cyclase identified to date, and triterpenes produced by this synthase include achilleol B (90%), tetracyclic (5.12%) and pentacyclic scaffolds (4.37%), and unidentified triterpenes (0.51%). The pathway for achilleol B biosynthesis is proposed. |
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