Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution |
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| Authors: | Rannes Julie B Ioannou Avgousta Willies Simon C Grogan Gideon Behrens Carsten Flitsch Sabine L Turner Nicholas J |
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| Affiliation: | School of Chemistry, University of Manchester, Manchester Interdisciplinary Biocentre, Manchester, M1 7DN, UK. |
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| Abstract: | A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. |
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