Interfacing matrix-assisted laser desorption/ionization mass spectrometry with column and planar separations |
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Authors: | Gusev A I |
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Institution: | Rohm and Haas Company, Research Laboratories, Spring House, PA 19477-0904, USA. arkady.gusev@rohmhaas.com |
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Abstract: | This paper presents a critical review of off-line and on-line coupling of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to liquid column separations (e.g. HPLC, GPC and CE) and planar separations (e.g. PAGE and TLC). Off-line MALDI analysis of fractions collected from HPLC, GPC and CE or spots scraped and extracted from TLC and PAGE has already become a routine practice for many laboratories. MALDI has also been used to obtain mass spectra directly from TLC plates and PAGE. The direct analysis methods range from dot-blotted samples to two-dimensional scanning of the entire gels/plates. Various combinations of on-line coupling of MALDI with column separations are also reviewed. The review discusses the strengths and limitations associated with different off-line and on-line coupling approaches. |
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