Computational modelling of amperometric biosensors in the case of substrate and product inhibition |
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Authors: | Dainius Šimelevičius Romas Baronas |
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Institution: | (1) Dept. Mathematics & Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania;(2) Dept. Chemistry & Bioengineering, Vilnius Gediminas Technical University, Sauletekio Ave. 11, LT-10223 Vilnius, Lithuania |
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Abstract: | In this paper the response of an amperometric biosensor at mixed enzyme kinetics and diffusion limitations is modelled in
the case of the substrate and the product inhibition. The model is based on non-stationary reaction–diffusion equations containing
a non-linear term related to non-Michaelis–Menten kinetics of an enzymatic reaction. A numerical simulation was carried out
using a finite difference technique. The complex enzyme kinetics produced different calibration curves for the response at
the transition and the steady-state. The biosensor operation is analysed with a special emphasis to the conditions at which
the biosensor response change shows a maximal value. The dependence of the biosensor sensitivity on the biosensor configuration
is also investigated. Results of the simulation are compared with known analytical results and with previously conducted researches
on the biosensors. |
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Keywords: | |
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