| Abstract: | Four procedures for the covalent immobilization of heparin (Hp) on a collagen film (CF) have been investigated. In three of them (methods (I–A, B, C), the CF was first treated with epichlorohydrin and ammonia and the Hp was added with the aid of CMBC (method I–A), by reductive amination in the presence of NaCH CN (method I–B), and with the aid of CMEC after succinylation (method I–C). In the fourth procedure (method II), the CF was activated by treatment with alkali and the Hp was added with the aid of CMEC. It was shown that the maximum amount of Hp was immobilized by method II. |
