Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific 15N-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10 |
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| Authors: | Xiaoqi Guo Bo Zhang Yao He Yangzhong Liu Changlin Tian |
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| Affiliation: | 1. High Magnetic Field Laboratory, Chinese Academy of Science, Hefei, and Hefei Natural Laboratory for Physical Science at Microscale, School of Life Science, University of Science and Technology of China, 230026, PR China;2. School of Chemistry and Material Science, University of Science and Technology of China, Hefei 230026, PR China |
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| Abstract: | The yield of unnatural amino acid o-nitrobenzyl-tyrosine (oNBTyr), which was able to recover to natural tyrosine after UV-photocleavage was greatly improved from 20% to 81% by using 2-nitrobenzyl bromide as the nucleophilic reagent. Through genetically introducing 15N-oNBY and consequent photo-cleavage, the site-specific 15N-Tyr NMR analysis of plant ABA (abscisic acid) receptor PYL10 was implemented without any residue variation. This isotope labelling of tyrosine onto protein backbone provides a convenient strategy for NMR analysis. |
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| Keywords: | Unnatural amino acid Tyrosine Protein NMR analysis Abscisic acid receptor PYL-10 |
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