Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis |
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Authors: | Yi Liu Xiaomei Ling Hui Fan Yingcheng Zhong Dan Li Ying Wang |
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Institution: | Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. |
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Abstract: | A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17x10(4) M(-1) and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively. |
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