高效液相色谱法同时测定化妆品中11种防腐剂

建立了化妆品中11种防腐剂HPLC测定方法。样品经甲醇超声提取后,经ZORBAX Extend XDB-C18柱分离,以甲醇-水（pH=8.0）为流动相,采用紫外检测器检测。11种防腐剂分离良好并排除了样品中杂质峰的干扰,各防腐剂在1—200mg/L范围内呈良好线性关系（r〉0.999）,平均回收率为93.4%—102.4%,相对标准偏差为0.81%—3.14%（n=5）,检出限为0.2—2.0mg/L。该方法可简便、快捷、准确的同时测定11种防腐剂。

Simultaneous Determination of Eleven Preservatives in Cosmetics by HPLC

An efficient method for analyzing eleven preservatives was developed by high performance liquid chromatography. Samples were extracted with methanol by ultrasonic, separated through ZORBAX Extend XDB-C_(18) column and determined with UV detector. Methanol-water(pH= 8.0) was used as mobile phase. Each preservative was separated without the interference from impurity. The linear correlation coefficient is (r) 0.999 in the range of 1-200mg/L. The average recoveries for the eleven preservatives are ranged from 93.4% to 102.4% with relative standard deviations of 0.81%-3.14%(n=5),and the limits of detection were from 0.2 to 2.0mg/L. The method is suitable to simultaneous assay the eleven preservatives in cosmetics with simplicity and accuracy in a single injection.