STUDY ON THE FUNCTIONAL SITES OF Mg~(2+) -INDUCED Chl α FLUORESCENCE AND SURFACE CHARGE CHANGES OF THYLAKOID MEMBRANES 期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

STUDY ON THE FUNCTIONAL SITES OF Mg~(2+) -INDUCED Chl α FLUORESCENCE AND SURFACE CHARGE CHANGES OF THYLAKOID MEMBRANES

作者姓名：	李良璧马红翟小京张正东马桂芝周佩珍

作者单位：	Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing，Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing，Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing，Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing，Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing，Laboratory of Photosynthesis，Institute of Botany，Academia Sinica，Beijing

摘要：	Thylakoid polypeptide components, Mg~(2+) -induced Chl a fluorescence at 77K and surface chargechanges were measured to investigate the functional sites of Mg~(2+) in the regulation of energy distri-bution between two photosystems in completely and incompletely developed barley chloroplasts. It wasfound that in contrast to the completely developed chloroplasts, the incompletely developed chloroplastslacked Chl b and did not contain the 23KDa and 25KDa polypeptide components of LHC-PSII. In themeantime, these membranes did nor present Mg~(2+) -induced Cbl a fluorescence and surface charge changesof thylakoids. These results provided strong evidence that the 23KDa and 25KDa polypeptides ofLHC-PSII are the specific acting sites of the cation that induced these two phenomena. It is suggested in this paper that Mg~(2+) -induced change of excitation energy distribution betweentwo photosystems is produced by the mechanism due to electrostatic neutralization of LHC-PSII bythe cation to cause a structural or con
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