| 反相流动注射化学发光法测定黄芩苷 |
| |
| 引用本文： | 林启凰,蔡怡珊,刘爱林,陈伟,黄金宝,廖靖萍,林新华.反相流动注射化学发光法测定黄芩苷[J].分析试验室,2010,29(3). |
| |
| 作者姓名： | 林启凰 蔡怡珊 刘爱林 陈伟 黄金宝 廖靖萍 林新华 |
| |
| 作者单位： | 1. 福建医科大学药学院药物分析学系,福州,350004
2. 福建国际旅行卫生保健中心,福州,350001 |
| |
| 基金项目： | 国家自然科学基金(20805006);;福建省科技重点基金(2008Y0045);;福建省医学创新课题(2009-CX-17);;福建省自然科学基金(C0710024);;博士启动基金(BS06002)项目资助 |
| |
| 摘 要： | 在碱性介质中,K3Fe(CN)6氧化鲁米诺产生化学发光,黄芩苷对该体系化学发光具有强烈的抑制作用。利用该化学发光的抑制体系,结合反相流动注射技术,建立了测定黄酮类药物黄芩苷含量的新方法。在优化的条件下,黄芩苷浓度在1.0×10-8～1.0×10-7和3.0×10-7～4.0×10-6mol/L范围内与化学发光抑制强度ΔI呈良好的线性关系,检出限为1.0×10-9mol/L,对3.0×10-7mol/L的黄芩苷进行平行测定10次,得相对标准偏差(RSD)为1.7%。该方法可应用于银黄口服液中黄芩提取物(黄芩苷计)的含量测定。
|
| 关 键 词： | 反相流动注射 化学发光 黄芩苷 银黄口服液 |
Determination of baicalin by a reversed-phase flow injection combined with chemiluminescence |
| |
| LIN Qi-huang,CAI Yi-shan,LIU Ai-lin,CHEN Wei,HUANG Jin-bao,LIAO Jing-ping,LIN Xin-hua.Determination of baicalin by a reversed-phase flow injection combined with chemiluminescence[J].Chinese Journal of Analysis Laboratory,2010,29(3). |
| |
| Authors: | LIN Qi-huang CAI Yi-shan LIU Ai-lin CHEN Wei HUANG Jin-bao LIAO Jing-ping LIN Xin-hua |
| |
| Institution: | 1.Department of Pharmaceutical Analysis of the Fujian Meidical University;Fuzhou 350004;2.Fujian International Travel Healthcare Center;Fujian Entry-Exit Inspection and Quarantine Bereau;Fuzhou 350001 |
| |
| Abstract: | A novel method for the determination of baicalin by flow injection chemiluminescence (FI-CL) system was presented. It was found that in alkaline medium, the CL intensity of the luminol-ferricyanide system could be inhibited in the presence of baicalin, and so we can detect the content of baicalin with the method. The proposed method was very sensitive, simple and quick. Under optimal conditions, the decrease of CL emission was correlated with baicalin concentration in the range from 1.0×10~(-8)to 1.0×10~(-7)and 3.0×10~(-7)to 4.0×10~(-6)mol/L. The detection limit was 1.0×10~(-9)mol/L, and the relative standard deviation is 1.7% for 3.0×10~(-7)mol/L baicalin solution in nine replicate measurements. The method has been successfully used to detect baicalin in the YinHuang oral liquid. |
| |
| Keywords: | Reversed phase flow injection analysis Chemiluminescence Baicalin Yinhuang oral liquid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
