Enhancement of the sensitivity and selectivity of oxidation of H2O2 on platinum wire at low working potential by platinization and covering of heteropolypyrrole film for amperometric micro-biosensor construction |
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| Authors: | Qing-Shan Li Bang-Ce Ye Bai-Xiang Liu Jian-Jiang Zhong |
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| Affiliation: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, 200237, P.R. China e-mail: jjzhong@ecust.edu.cn, CN;(2) Research Institute of Biochemical Engineering, East China University of Science & Technology, Shanghai, 200237, P.R. China, CN;(3) Research Institute of Biochemistry, East China University of Science & Technology, Shanghai, 200237, P.R. China, CN |
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| Abstract: | A microelectrode for glucose determination was constructed by immobilization of glucose oxidase (GOx) on a platinized platinum (Pt) by electrochemical polymerization of a solution containing GOx, pyrrole, and a substituted pyrrole, 4-(3-pyrrolyl)-4-oxobutyric acid. Due to platinization and covering with the polymerized heteropolypyrrole (hPPy) film, the electrode prepared showed high sensitivity to H2O2 at a low potential and significantly reduced the response to electroactive compounds, such as ascorbate, urate and 4-acetamidophenol. Working at 200 mV (vs. SCE) the electrode showed a linear response to glucose from 1.6 to 10 mM with a high sensitivity of 1 μA/mM, whereas the response to 1 mM ascorbate, urate, and 4-acetamidophenol was 0.53 μA, 18 nA and 4 nA, respectively, which was about 2.5%, 1.0% and 1.0% of that at a bare electrode. The stability of the electrode was tested at intervals of three or five days, and each test lasted about two hours. After 6 months examination, only 30% of its activity was lost. Received: 16 March / Revised: 26 July 1998 / Accepted: 1 August 1998 |
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