A novel piezoelectric biosensor for the detection of phytohormone beta-indole acetic acid. |
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| Authors: | Jin Li Zhao-Yang Wu Lang-Tao Xiao Guang-Ming Zeng Guo-He Huang Guo-Li Shen Ru-Qin Yu |
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| Affiliation: | State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University, ChangSha, P R China. |
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| Abstract: | A novel piezoelectric immunosensor has been developed for the determination of beta-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 microg/ml with a regression equation of the form y = -23x + 151 (r = 0.9937). |
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