中药复方和某种植物分泌液对脂质过氧化的抑制作用

利用发光测量法,研究了中药复方、植物分泌液(SL)和植物分泌液复方(SLIV)对Fe2+-亚麻油酸-BSA(牛血清白蛋白)和Fe2+-脑勺浆两个离体体系产生的脂质过氧化(LPO)有抑制作用,抑制程度与用药浓度有关.将中药复方应用于辐照模型,结果表明7Gy的γ射线辐照后小白鼠全血硒谷胱甘肽过氧化物酶(SeGSH-Px)活性降低,恢复缓慢,脑组织脂质过氧化加剧,而事先喂饲中药复方能显著提高SeGSH-Px水平,加速其活力恢复,增强脑组织抗氧化能力;将SL和SLIV应用于脑缺血重灌模型,结果显示沙土鼠脑缺血重灌后全血SeGSH-Px活力下降,血浆MDA上升,SeGSH-Px/MDA值降低,脑组织抗氧化能力下降,而事先喂饲SL、SLIV上述结果正相反,能明显提高机体的抗LPO能力.

THE PROPERTIES OF CHM, SL AND SLIV TO INHIBIT LIPID PEROXIDATION

In this paper,the capabilities of CHM,SL and SLIV to inhibit lipid peroxidation(LPO)in vitro and in vivo have been studied.In vitro,the concentration-dependent decreases of ultraweak chemiluninescence were observed when CHM,SL and SLIV were added to both Fe2+-lindenic Acid-BAS and Fe2+-Brain Homogenate system respectively.In vivo,CHM may contribute to the reinforcement and recovery of antiperoxidation abilities of γ-ray-treated mice by increasing the activity of SeGSH-Px.SL and SLIV can protect ischemic-reperfused Mongolian Gerbil against LPO injuries by enhancing the value of SeGSH-Px/MDA.CHM,SL and SLIV also can protect brain tissue against LPO in vivo,shown as lower level of brain homogenate natural or induced chemiluminescence.