<非洲绿猴和食蟹猴主要肠道细菌的实时定量 PCR 检测及分析

摘要:目的 非洲绿猴具有不同于食蟹猴和恒河猴等猕猴的生物学特征,如脂质代谢、自发性高血压、SIV 感染不引起获得性免疫缺陷综合症( AIDS)等,本研究拟对食蟹猴和非洲绿猴肠道细菌的相对含量进行比较研究。 方法随机选取 SIV 抗体阴性的成年食蟹猴和非洲绿猴各 12 只,排除肥胖和疾病个体,收集其新鲜粪便样本,按 200 mg每份等分后进行液氮速冻,-80 ℃ 保存。 其中 1 份进行肠道细菌的实时定量 PCR 分析。 结果 与食蟹猴相比,非洲绿猴双歧杆菌、乳酸菌以及肠杆菌相对含量显著偏低( P<0. 05) ,而普氏菌以及丁酸盐产生菌 ———普拉梭菌和真细菌相对含量更高( P<0. 05) 。 结论 非洲绿猴产丁酸盐的细菌相对含量更高,反映出其肠道免疫细胞稳态和黏膜完整性具有不同于食蟹猴的进化适应特征,为深入研究非洲绿猴对 SIV 感染不致病的相关机制奠定了基础。

Real-time PCR Detection and Analysis of the Gut Microbiota in African Green Monkey and Cynomolgus Macaque

Abstract:Objective As a SIV natural host, African green monkey ( AGM ) shows AIDS resistance. Also, spontaneous hypertension develops in AGM. Different capacities in lipid metabolism between AGM and Cynomolgus macaque ( CM) exist. This study aims to characterize the gut microbiome profiles of AGM and CM. Method Fecal samples were collected for 12 AGM and 12 CM. Real-time PCR detection of the main gut bacteria in AGM and CM was performed and analyzed. Result Our results demonstrated a lower abundance of Bifidobacteria and a higher abundance of Prevotella in AGM. Compared with CM, AGM possessed a higher abundance of the butyrateproducing Faecalibacterium prausnitzii and Eubacteria ( P < 0. 05 ) . Conclusion Compared with CM, the gut microbiota of AGM resembles a trend as found in early SIV infection or chronic HIV infection. AGM possesses a relative higher abundance of the butyrate-producing gut bacteria which correlates with gut homeostasis and mucosal integrity. These findings may provide insight into environmental adaption and AIDS resistance in AGM.