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| 紫外分光光度法测定大豆中大豆异黄酮 | |
| 引用本文： | 魏福华,张永忠,井乐刚,王继安. 紫外分光光度法测定大豆中大豆异黄酮[J]. 理化检验(化学分册), 2006, 42(6): 461-463 |
| 作者姓名： | 魏福华 张永忠 井乐刚 王继安 |
| 作者单位： | 1. 江苏食品职业技术学院,淮安,223003 2. 哈尔滨师范大学,阿城学院生物系,阿城,150301 3. 东北农业大学,大豆研究所,哈尔滨,150030 |
| 摘 要： | 以染料木苷为标准品,利用三波长紫外分光光度法对东北大豆的11个品种进行了异黄酮含量的测定。测定结果表明,“垦丰”和“中豆”两个品种的异黄酮含量较高,分别达到0．246％和0．221％;“东农44单株”和“东农355”两个品种的异黄酮含量较低,分别为0．101％和0．110％。 |
| 关 键 词： | 紫外分光光度法 染料木苷 大豆异黄酮 |
| 文章编号： | 1001-4020（2006）06-0461-03 |
| 收稿时间： | 2004-07-28 |
| 修稿时间： | 2004-07-28 |
UV-SPECTROPHOTOMETRIC DETERMINATION OF ISOFLAVONES IN SOYBEAN PRODUCED IN NORTHEAST REGION OF CHINA |
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| WEI Fu-hua,ZHANG Yong-zhong,JING Le-gang,WANG Ji'an. UV-SPECTROPHOTOMETRIC DETERMINATION OF ISOFLAVONES IN SOYBEAN PRODUCED IN NORTHEAST REGION OF CHINA[J]. Physical Testing and Chemical Analysis Part B:Chemical Analgsis, 2006, 42(6): 461-463 | |
| Authors: | WEI Fu-hua ZHANG Yong-zhong JING Le-gang WANG Ji'an |
| Abstract: | The application of triple-wavelength spectrophotometry to the determination of isoflavones in soybean,eliminates interferences of co-existing substances effectively,and gives more reliable analytical results which make the comparative study to have a more reasonable ground.Genistin was used as standard in the UV-spectrophotometric determination,which gave more accurate and precise results as compared with the standard conventionally used in literature.Eleven different species of soybean samples from the northeast region were analyzed by this method,and as shown by the analytical results, two of the species,i.e.the "Kenfeng" and "Zhong dou",contain higher contents of isoflavones(0.246% and 0.221% respectively),and two other species i.e. the "Dongnong 44 danzhu" and "Dongnong 355",contain lower contents of isoflavones(0.101% and 0.110% respectively). |
| Keywords: | UV-spectrophotometry Soybean isoflavones C-enistin |
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