Allosterically activated Diels-Alder catalysis by a ribozyme |
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Authors: | Helm Mark Petermeier Markus Ge Bixia Fiammengo Roberto Jäschke Andres |
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Affiliation: | Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany. |
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Abstract: | We describe the allosteric control of Diels-Alder reactions by a small organic effector, theophylline. This is achieved by converting a Diels-Alder ribozyme into an allosterically regulated system. In contrast to other published systems, we have a bond-forming reaction with two small-molecule substrates and multiple turnover. This system could be very attractive for the development of assays for a variety of analytes and can be regarded as a prototype of fully synthetic signaling cascades. |
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