| HPLC-ELSD法测定绞股蓝中绞股蓝皂苷A的含量 |
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| 引用本文： | 乐圆,肖娅萍,张宁,洪叶岚,薛婧. HPLC-ELSD法测定绞股蓝中绞股蓝皂苷A的含量[J]. 光谱实验室, 2010, 27(5): 1734-1737 |
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| 作者姓名： | 乐圆 肖娅萍 张宁 洪叶岚 薛婧 |
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| 作者单位： | 教育部药用植物资源及天然药物化学重点实验室,西安市长安南路199号,710062;陕西师范大学生命科学学院,西安市长安南路199号,710062;教育部药用植物资源及天然药物化学重点实验室,西安市长安南路199号,710062;西部濒危药材开发国家工程实验室,西安市长安南路199号,710062;陕西师范大学生命科学学院,西安市长安南路199号,710062 |
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| 基金项目： | 十一五科技支撑计划项目 |
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| 摘 要： | 采用HPLC-ELSD法测定绞股蓝中绞股蓝皂苷A的含量。色谱柱为HibarC18(250×4.6mm,5μm),流动相为乙腈-水梯度洗脱,流速0.5mL/min,柱温为20℃,绞股蓝皂苷A在0.0425—1.995mg/mL范围内线性关系良好(r2=0.9962),并通过正交法优化其提取工艺。该方法简便、有效,为绞股蓝的质量控制提供一种准确可行的检测方法。
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| 关 键 词： | 绞股蓝 绞股蓝皂苷A 高效液相色谱-蒸发光散射检测器 正交实验 |
Determination of Gypenoside A in Gynostemma Pentaphyllum(Thunb.) Makino by HPLC-ELSD |
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| LE Yuan,XIAO Ya-Ping,ZHANG Ning,HONG Ye-Lan,XUE Jing. Determination of Gypenoside A in Gynostemma Pentaphyllum(Thunb.) Makino by HPLC-ELSD[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(5): 1734-1737 |
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| Authors: | LE Yuan XIAO Ya-Ping ZHANG Ning HONG Ye-Lan XUE Jing |
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| Affiliation: | a,ca(Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi＇an 710062,P.R.China)b(National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,Xi＇an 710062,P.R.China)c(College of Life Science,Shaanxi Normal University,Xi＇an 710062,P.R.China) |
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| Abstract: | Gypenoside A in Gynostemma pentaphyllum（Thunb.）Makino was determined by HPLC-ELSD.The separation was performed on Hibar C18 column（250×4.6mm,5μm）,by gradient elution using acetonitrile-H2O as the mobile phase at the flow rate of 0.5mL/min,and the temperature of column was 20℃.The linear range of gypenoside A was 0.0425—1.995mg/mL（r2=0.9962）.Also,the extraction was studied by the orthogonal experiment.This method was simple,effective and feasible,and can be used to control the quality of Gynostemma pentaphyllum（Thunb.）Makino. |
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| Keywords: | Gynostemma Pentaphyllum（Thunb.）Makino Gypenoside A HPLC-ELSD Orthogonal Experiment |
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