Isotachophoresis of proteins in uncoated open-tubular fused-silica capillaries with a simple approach for column conditioning.

The isotachophoretic determination of proteins in uncoated open-tubular fused-silica capillaries of 50 and 75 microns I.C. with on-column multi-wavelength detection is reported. Small amounts of hydroxypropylmethylcellulose added to the leader provide an efficient method of dynamic column conditioning which allows the high-resolution isotachophoretic determination of most proteins to be performed in the presence of an electro-osmotic flow. Different approaches for cationic and anionic analyses are discussed and illustrated with selected examples.