Isolation of Catharanthus alkaloids by solid-phase extraction and semipreparative HPLC |
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| Authors: | T Naaranlahti M Nordstr?m S P Lapinjoki A Huhtikangas M Lounasmaa |
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| Institution: | Department of Pharmaceutical Chemistry, University of Kuopio, Finland. |
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| Abstract: | A combination of moderate-pressure chromatography on C18 sorbent and preparative HPLC is developed for rapid isolation of alkaloids from Catharanthus roseus. The procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. The methodology is also applied for identification of the above and other alkaloids from cultured plant cells. |
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