Highly Selective and Sensitive Near‐Infrared‐Fluorescent Probes for the Detection of Cellular Hydrogen Sulfide and the Imaging of H2S in Mice |
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| Authors: | Dr. Haixia Wu Saarangan Krishnakumar Jie Yu Dong Liang Prof. Dr. Hongyi Qi Zheng‐Wei Lee Prof. Dr. Lih‐Wen Deng Prof. Dr. Dejian Huang |
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| Affiliation: | 1. Food Science and Technology Programme, Department of Chemistry, National University of Singapore, Science Drive 3, 117543 (Singapore);2. College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715 (China);3. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 8 Medical Drive, 117597 (Singapore);4. National University of Singapore (Suzhou) Research, Institute, 377 Lin Quan Street, Suzhou Industrial Park, Jiangsu, 215123 (China) |
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| Abstract: | Herein, we report the development of two fluorescent probes for the highly selective and sensitive detection of H2S. The probes take advantage of a CuII? cyclen complex, which acts as a reaction center for H2S and as a quencher of BODIPY (boron‐dipyrromethene)‐based fluorophores with emissions at 765 and 680 nm, respectively. These non‐fluorescent probes could only be turned on by the addition of H2S, and not by other potentially interfering biomolecules, including reactive oxygen species, cysteine, and glutathione. In a chemical system, both probes detected H2S with a detection limit of 80 nM . The probes were successfully used for the endogenous detection of H2S in HEK 293 cells, for measuring the H2S‐release activity of dietary organosulfides in MCF‐7 cells, and for the in vivo imaging of H2S in mice. |
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| Keywords: | copper fluorescence hydrogen sulfide imaging agents probes |
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