复方丹参片生产中丹酚酸B的快速测定

为建立制药企业在生产复方丹参片时的快速质量控制方法,用TLC及反相HPLC法,对在不同处理条件下的复方丹参片样品中丹酚酸B进行了定性、定量分析,同时优化了色谱条件,提高了测定的准确性。结果表明,甲醇超声处理复方丹参片粉末20 min(1次),再用高纯水超声处理20 min(2次);流动相V(甲醇) V(乙腈) (V甲酸) V(水)=25 10 1 64,波长286 nm。本法线性好,准确度高,简便快捷经济,可用于生产该药的内在质量监测。

Rapid Analysis on Salvianolic Acid B in Compound Tanshen Pian

To establish an method of rapid quality monitoring for compound Tanshen pian in pharmacy,through optimizing extraction technique and chromatography conditions,to analyze the salvianolic acid B in compound Tanshen pian about qualitative and quantitative by TLC and HPLC;besides,and the accuracy of quantitative analys is was improved.The results show that the dispose of compound Tanshen pian is ultrasonic extraction,at frost with methanol for 20 min and then with water at twice for 20 min,mobile phase: V(methanol) V(acetonitrile) V(methanoic acid) V(water) = 25 10 1 64,the measure wavelength: 286 nm.It concludes the methods are simple,rapid,economical,sensitive and accurate.The liner rang is good(r = 0.999 4),it can be used to control the quality of compound Tanshen pian in medicine company.