Determination of histamine in port wines and grape juices by ion-pair extraction and stable isotope dilution GC-MS |
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Authors: | J O Fernandes M A Ferreira |
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Institution: | (1) CEQUP/Laboratório de Bromatologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 4050 Porto, Portugal |
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Abstract: | Summary An isotope dilution, GC-MS method for the quantitation of histamine in wines and grape juices was assayed. This method includes
isolation of histamine and its, deuterated analogue α,α,β,β-2H4]histamine, used as the labelled internal standard, by ion-pair extraction with bis-2-ethylhexylphosphate, conversion to the
volatile derivative: tris-pentafluorobenzyl-histamine, capillary GC separation and measurement of the abundance ratio of the
(M-181)+ ions from labelled and unlabelled derivatives.
The method was successfully applied to the determination of histamine in several samples of Port wine, red and white table
wine and grape juice. Data are given on detectability, linearity, repeatability and recovery. |
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Keywords: | Gas chromatography-mass spectrometry Isotope dilution Ion-pair extraction Histamine Port wine |
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