Abstract: | An affinity or biospecific method of isolating an iron-binding glycoprotein — lactoferrin — from breast milk is described which permits in a single chromatographic operation the isolation of lactoferrin homogeneous according to the results of electrophoresis in polyacrylamide gel. The affinity method of isolating lactoferrin is based on the use of a specific immunosorbent consisting of antibodies to lactoferrin covalently conjugated with cyanogen-bromide-activated Sepharose 4B.Scientific-Research Institute of Hematology and Blood Transfusion Ministry of Health of the Uzbek SSR, Tashkent. Translated from Khimiya Priordnykh Soedinenii, No. 6, pp. 749–750, November–December, 1986. |