Chemiluminescence determination of tiopronin and its metabolite 2-mercaptopropionic acid in human urine by HPLC coupled with flow injection |
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| Authors: | Yining Zhao W R G Baeyens X R Zhang A C Calokerinos K Nakashima G Van Der Weken A Van Overbeke |
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| Institution: | (1) Faculty of Pharmaceutical Sciences, Laboratory of Drug Quality Control, University of Ghent, Harelbekestraat 72, 9000 Ghent, Belgium;(2) Chemistry Department, Laboratory of Analytical Chemistry, University of Athens, Panepistimiopolis 15771, Athens, Greece;(3) School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852 Nagasaki, Japan |
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| Abstract: | Summary In previous pharmacokinetic studies tiopronin, a drug used for effective treatment of cystinuria and rheumatoid arthritis,
and its metabolite 2-mercaptopropionic acid were analysed by conventional liquid chromatography with pre- and post-column
derivatization and UV detection. Now a novel HPLC-coupled chemiluminescence-flow-injection analysis (CL-FIA) method has been
developed for the determination of tiopronin and 2-mercaptopropionic acid in urine. The method is based on chemiluminescence
from a Ce(IV) oxidation system sensitized by quinine, as proposed earlier by this group, and flow-injection analysis. The
method, which has the advantages of high sensitivity and selectivity, simple sample treatment and prompt production of results,
has also been preliminarily adapted for pharmacokinetic study of tiopronin in urine. |
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| Keywords: | Column liquid chromatography Chemiluminescence detection Tiopronin Thiols |
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