Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography |
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Authors: | Zbrozek Jaroslav Pumera Martin Flegel Martin |
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Institution: | Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague. |
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Abstract: | Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent. |
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